Activation of CgOPT1 transcription by IAA and during spore germination. A. Spores were germinated in pea extract and CgOPT1 expression was determined at various time points. Top – CgOPT1, bottom – rRNA. B. Expression of CgOPT1 in mycelia was determined after growing the fungus for 48 h in CD medium (0), CD supplemented with 500 μM tryptophol (Tol), or CD with 100 μM or 500 μM IAA. Top – CgOPT1, bottom – rRNA. C. The transgenic strain Pop-gfp6 was grown in CD media supplemented with various concentrations of IAA. GFP levels were evaluated 48 h after culture inoculation. Control (0) contained an equal volume of ethanol. Low magnification image is presented as inset in each frame. The portion of the colony that is presented in higher magnification is designated by a small square within each inset. Bars = 20 μm.