Skip to main content

Advertisement

Table 2 MIC results and Erg11p substitutions for 25 C. albicans isolates with reduced susceptibility to fluconazole and 23 fluconazole-susceptible isolates by RCAa and ERG11 sequencing.

From: Rapid detection of ERG11 gene mutations in clinical Candida albicans isolates with reduced susceptibility to fluconazole by rolling circle amplification and DNA sequencing

   MIC (μg/ml) Erg 11p amino acid substitutions
     D D E F F G G G G K K R S V V Y
     1 2 2 1 4 3 4 4 4 1 1 4 4 4 4 1
Patient/isolate no. Site FLU a VOR a 1 7 6 4 4 0 4 6 6 2 4 6 0 3 8 3
     6 8 6 5 9 7 8 4 5 8 3 7 5 7 8 2
     E E D L S S E S S T R K F I I H
Isolates with reduced fluconazole susceptibility
1b Oropharynx 16 0.25    + +            +  
2b Vagina >256 0.03                 
3-Ab, c Oropharynx 16 0.25    + +            +  
- Bb, c Oropharynx 16 0.5    + +            +  
4d Oropharynx 256 0.25    +         +     +  
5d Oropharynx 256 0.125       +           
6-Ac, d Oropharynx 256 >16    +              
-Bc, d Oropharynx 256 >16 +                
7d Oropharynx 256 >16    +      +    +     +  
8-Ac, d Oropharynx 256 0.5    +   +           +  
-Bc, d Oropharynx 256 1    +   +           +  
9d Oropharynx 256 >16    +            +   
10d Oropharynx 256 2    +           +   + +
11d Oropharynx 256 0.008    +            +   
12-Ac, d Oropharynx 256 2         +         
-Bc, d Sputum 32 0.5 +        + +        
13d Vagina 32 0.016           +       
14d Blood 256 >16 +          +       
15d Bile 256 16               +   
16-Ac, d Oropharynx 16 0.125 +       +    +       
-Bc Oropharynx 256 2 +      + +    +       
-Cc, d Oropharynx 256 16 +      + + +   +       
17d Oropharynx 64 0.5   +               
18d Oropharynx 128 2 +          +   +     
19d Oropharynx 256 0.5 +          + +      
Fluconazole-susceptible isolates b
ATCC 10231 UNa 0.125 0.008                 
ATCC 90028 Blood 025 0.03                 
20 Blood 0.25 <0.008    +            +   
21 Oropharynx 0.12 0.008 +   +              
22 Blood 0.5 0.016                 
23 Blood 0.5 0.016                 
24 Blood 0.25 0.008                 
25 Blood 0.25 0.008    +             +  
26 Blood 0.5 0.008 +   +              
27 Blood 0.5 0.008 +   +              
28 Blood 0.25 <0.008    +            +   
29 Skin 1 0.016 +          +       
30 Peritoneal fluid 1 2 +   +              
31 Blood 0.12 0.125 +   +             +  
32 Blood 0.125 0.008 +          +       
33 Blood 0.5 0.008 +   +             +  
34 Tissue 0.25 0.008 +          +       
35 Blood 2 0.008    +             +  
36 Blood 0.25 0.016    +             +  
37 Liver 0.5 <0.008    +             +  
38 Blood 0.25 <0.008 +   +             +  
39 Blood 0.125 <0.008    +              
40 Bone 0.25 <0.008 +   +              
  1. The "+" sign denotes the presence of the mutation.
  2. aAbbreviations: RCA, rolling circle amplification; FLU, fluconazole; VOR, voriconazole; UN, unknown.
  3. b Isolates from the Centre for Infectious Diseases and Microbiology, Westmead Hospital, Sydney.
  4. c The "A" and "B" notation of patient numbers refers to isolates cultured sequentially from the same patient at different times.
  5. dIsolates obtained from the Mycology Unit, Women's and Children's Hospital, Adelaide.