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Table 2 Kinetic analysis of wild type and mutant LadA

From: A single amino acid change (Y318F) in the L-arabitol dehydrogenase (LadA) from Aspergillus niger results in a significant increase in affinity for D-sorbitol

  Wild type Y318F
  Km Vmax Kcat Km Vmax Kcat
L-arabitol 0.056 96.2 863 0.078 176.8 1800
Xylitol 0.250 131.5 1180 0.218 216.8 2208
D-sorbitol 4.122 90.2 809 0.868 81.8 833
  1. ND = not determined.