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Table 2 Kinetic analysis of wild type and mutant LadA

From: A single amino acid change (Y318F) in the L-arabitol dehydrogenase (LadA) from Aspergillus niger results in a significant increase in affinity for D-sorbitol

 

Wild type

Y318F

 

Km

Vmax

Kcat

Km

Vmax

Kcat

L-arabitol

0.056

96.2

863

0.078

176.8

1800

Xylitol

0.250

131.5

1180

0.218

216.8

2208

D-sorbitol

4.122

90.2

809

0.868

81.8

833

  1. ND = not determined.
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BMC Microbiology

ISSN: 1471-2180

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