Rapid identification of bacterial pathogens using a PCR- and microarray-based assay

BMC Microbiology

Table 1 Oligonucleotide probes included in the final microarray layout.

Targeted bacteria	Sequence (5'->3')	Length	T_m (°C)
Acinetobacter baumannii	AGTGTTTCAGATAATGGCC	19	47
	AATTATTGTCACGATTCACGAGG	23	52
Enterococcus faecalis	CTGGTATCCCAACAGTAGAAGTA (parE)	23	53
Enterococcus faecium	TATCACCGTTATTGACGACGGTC	23	55
	GGATACCTGTAGATATCCAGGCA	23	55
Haemophilus influenzae	GGCCATTGTTCCGATATTATCG	22	53
	GTTCCGATATTATCGTGACAA	21	49
Klebsiella pneumoniae	TACTGCAAAGATATCGTTGTCA	22	49
Listeria monocytogenes	TACAATCGAAGCTGATAACAGCA	23	52
	ACTGTTCGTGATAACGGACGTGG	23	57
	GGTCGTCCAACAGTAGAAGT	20	52
Neisseria meningitidis	AATCACGGTAACGATACACGC	21	52
Staphylococcus aureus	CTGTCGAAGTTATTTTAACTGTTT	24	49
	AATAGTATCGATGAAGCATTAGCTG	25	53
Staphylococcus epidermidis	TAGTCATATTGAAGTTRTAATTGAG	25	48–49
	CATTAGCAGGTTATGCTAGTCATA	24	52
CNS	TCAACTTCAGAAAAAGGTTTACA	23	48
	CGCCCAGCAGTTGAAGTTATCT	22	55
Streptococcus agalactiae	TTACATTGAACCAGATAACTCTA	23	48
	TGGAAGACCAGCTGTAGAGACAG	23	57
Streptococcus pneumoniae	TGGTGATCGTATTGATGTAACTA (parE)	23	50
Streptococcus pyogenes	GTCCCGCCGTTGAAACAGTT	20	54
	TTTTTACAGTCTTACACGCAGGT	23	52
	GCAGGTTTTGCCTCTCATATTAAAGTCTT	29	57
mecA	TGATTATGGCTCAGGTACTGC	21	52
	TGGCTCAGGTACTGCTATCCA	21	54
	ATTAGCACTTGTAAGCACACC	21	50
	TACTGCTATCCACCCTCAAAC	21	52

If not otherwise stated, the presented sequence targets the gyrB gene.
Melting temperature (T_m, basic) is calculated using software available at http://www.basic.northwestern.edu/biotools/oligocalc.html.

ISSN: 1471-2180