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Table 3 Plasmids used in this study

From: Cj0596 is a periplasmic peptidyl prolyl cis-trans isomerase involved in Campylobacter jejuni motility, invasion, and colonization

Plasmid Description Source
pET-20b(+) Cloning vector, AmpR Novagen
pCR II-TOPO Cloning vector, KanR AmpR Invitrogen
pKR001 cj0596 gene and flanking region in pCR II-TOPO This study
pKR002 Self-ligated inverse PCR product of pKR001 with cj0596 removed This study
pKR016 cj0596 gene lacking signal sequence and stop codon in pCR II-TOPO This study
pKR017 cj0596 gene lacking signal sequence and stop codon in pET-20b(+) This study
pKR018 cat cassette in pCR II-TOPO This study
pKR019 H. pylori 84–183 rpsL gene in pCR II-TOPO This study
pKR020 cat cassette in pKR002 This study
pKR021 rpsL HP /cat construct in pKR002 This study