Biofilm formation by Mycobacterium avium isolates originating from humans, swine and birds

BMC Microbiology

Table 4 Presence of genes related to glycopeptidolipid synthesis, biofilm-formation, RFLP-clustering, presence of ISMpa1 and hsp65-code among Mycobacterium aviumisolates.

Isolates	Origin	Relation¹	ISMpa1	hsp65	nsGPL genes²	ser2genes³
Group I
989	Bird		-	-	+	+
1553,1794	Bird		-	4	+	+
ATCC 25291	Ref str.		-	-	+	+
R13	Ref str.		-	4	+	+
H31	Human		-	-	+	+
H38	Human		-	3	+	+
VI105	Swine	H15	-	1	+	+
1572, 1573, VI133	Swine		-	3	+	+
1577	Swine	1668	-	3	+	+
1606	Swine	1573	-	3	+	+
1851	Swine	VI133	-	3	+	+
Group II
H21, H22	Human		-	-	+	-
H12, H15, H28	Human		-	1	+	-
H1	Human		-	2	+	-
H17	Human		-	3	+	-
1555, 1628	Swine		-	-	+	-
1603	Swine		+	-	+	-
1668, 1831	Swine		-	-	+	-
1838	Swine	1876	-	-	+	-
1646	Swine	989	-	1	+	-
1876, 1878	Swine		-	1	+	-
VI101	Swine	H21	-	3	+	-
VI104	Swine	H17	-	3	+	-
Group III
1655, 1591	Swine		+	-	-⁴	-
1649, 1802	Swine		+	-	-	-
1656	Swine	1670	+	-	-	-
1627, 1638, 1670	Swine		+	1	-	-

The isolates are divided into three groups, I, II and III, based on presence or absence of GPL genes.
¹Isolate related by RFLP (Figure 1)
²nsGPL genes: gtfA, rtfA and mtfC
³ser2 genes: mdhtA, merA and mtfF
⁴1591 and 1655 had a weak PCR product for mtfC. Sequencing showed a product with few bases different from AF125999 TMC724/ATCC 25291). The PCR product of #1591 was identical to the sequence of the mtfC gene of M. avium 104
Biofilm forming isolates are marked in bold typing.

ISSN: 1471-2180