Comparison between M. perniciosa aegerolysins and other fungi. A – Alignment for similarity between ORFs of the two probable aegerolysins of M. perniciosa (MpPRIA1 and MpPRIA2) and aegerolysins of M. perniciosa FA553 (gbEEB90416.1), A. aegerita (spO42717.1), P. ostreatus (PriA – gbAAL57035.1 and ostreolysin – gbAAX21097.1), A. fumigatus Af293 (XP 748379.1), A. fumigatus (gbBAA03951.1) C. immitis RS (XP_001242288.1) A. niger (XP_001389418.1). Strictly conserved residues are shown in black and similar residues in gray. Consensus symbols: ! is any of IV, $ is any of LM, % is any of FY, # is any of NDQEBZ. Domain PF06355 (aegerolysin family) is present in MpPRIA1 (residues 4–136, score 8.7e-61) and MpPRIA2 (residues 29–135, score 4.2e-34). B. Phylogenetic analysis of the probable aegerolysin genes of M. perniciosa with above-cited sequences. Evolutionary history was inferred using the Neighbor-Joining method. The bootstrap consensus tree inferred from 1000 replicates is taken to represent the evolutionary history of the analyzed taxa.