Mycelial stages prior to emergence of M. perniciosa primordia. A, B, C. Mycelial mat originating from basidiospore germination on dead cocoa branches. D. Mycelial mat cultured on artificial substrate. Mycelium is initially white (A) then turns yellow (B) and changes to reddish pink (C) (A, B, C; bars = 0.5 cm), and maintains this color during primordial and basidiomata development, both in natural and artificial conditions (D; bar = 1.25 cm). E. Globose protuberance covered by mycelial mat (*) and openings for initial sprouting (bar = 1 mm). F. Primordia emergence (bar = 1 mm). G. Schematic representation of the sampling during cultivation for library construction (CP03) and macroarrays and RT-qPCR (CP02). Lateral numbers indicate days of cultivation. Box A – time 0, when the Petri dishes were inoculated. Box B – First harvest before hanging the mycelia in moist growth chambers. Box C – Second harvest with yellow mycelia. Box D – Third harvest with pink-reddish mycelium. Box E – Fourth harvest with reddish-pink mycelium before stress. Box F – Fifth harvest with dark pink mycelia (CP03), or reddish-pink after stress (CP02). G – Sixth harvest of primordia and fully-developed basidiomata. The days of cultivation differ due the differences between fungal isolates.