Effect of silencing An. gambiae (G3) CTL4, LRIM1, TEP1, or LRIM2 on P. yoelii infection. The images illustrate the level of infection and parasite melanization observed 6 days post infection (PI) when each gene was silenced. Live parasites are detected with green fluorescence (left panels) and those melanized are in DIC images (right panels). Effect of silencing C-type lectin 4 (CTL4) (Panel A), leucine rich-repeat immune protein 1 (LRIM1) (Panel B), thioester-containing protein 1 (TEP1) (Panel C), or leucine rich-repeat immune protein 2 (LRIM2) (Panel D) on P. yoelii infection. The dots represent the number of live (green) or melanized (black) parasites on individual midguts 6 days PI. The median number of oocysts is indicated by the horizontal line. Distributions are compared using the Kolmogorov-Smirnov test; n = number of mosquitoes; P values lower than 0.05 are consider to be significantly different.