Figure 3

Susceptibility of An. stephensi (Nijmegen Sda500) and An. gambiae (G3) to P. yoelii infection. An. stephensi and An. gambiae mosquitoes were fed on the same P. yoelii-infected mouse. The images illustrate the level of infection and parasite melanization observed 6 days post infection (PI) in An. stephensi (Panel A) or An. gambiae (Panel B) females infected with P. yoelii. Live parasites are detected with green fluorescence (left panels), and those melanized are in DIC images (right panels). Panel C, Number of live (green dots) or melanized (black dots) parasites present on individual midguts 6 days PI. The median number of oocysts is indicated by the horizontal line. Distributions are compared using the Kolmogorov-Smirnov test; n = number of mosquitoes; P values lower than 0.05 are consider to be significantly different. Panel D, The number of live (green dots) and melanized (black dots) P. yoelii parasites on individual An. gambiae midguts is shown connected by a line. In most mosquitoes, either all parasites are alive or all are melanized. There are very few midguts in which both live and melanized parasites are observed.