Effect of silencing An. gambiae (G3) GSTT1 and GSTT2 on P. berghei infection. Panel A, Effect of silencing glutathione-S-transferase theta-1 (GSTT1) on Plasmodium infection. GFP-expressing parasites were counted directly 6 days post infection (PI). Panel B, Effect of silencing glutathione-S-transferase theta-2 (GSTT2) on Plasmodium infection. Infection levels were determined based on the relative abundance of P. berghei 28S and An. gambiae S7 genes in genomic DNA isolated from midguts 6 days PI. The dots represent the infection level on individual midguts, and the median infection level is indicated by the horizontal line. Distributions are shown using a logarithmic scale for GSTT2 and are compared using the Kolmogorov-Smirnov (KS) test; n = number of mosquitoes; P values lower than 0.05 are considered to be significantly different.