PipB-mediated entry of SE into COEC and suppression of AvBDs in SE-infected COEC. COEC in 48-well culture plates were infected with ZM100 (wt), ZM106 (pipB), or ZM106-C (pipB, pPipB) at MOI of 20:1 (bacteria:cell). Data shown are geometric means of three independent experiments ± standard deviation. 6A. Number of intracellular bacteria (log CFU/well) at 1 hpi. * indicates that the difference in the number of intracellular bacteria between ZM100 (wt) and ZM106 (pipB) is significant (p < 0.05). 6B. SE-induced changes in the mRNA expression of AvBDs in COEC at 1 hpi. * indicates that the difference between the amounts of AvBD transcripts in ZM100-infected COEC and ZM106-infected COEC is significant (p < 0.05).