Expression patterns of 14 known AvBDs in COEC. Total RNA extracted from uninfected COEC was reverse transcribed into cDNA and amplified using primers specific for β-actin and individual AvBD genes. 2A. RT-PCR products subjected to 1.5% agarose gel electrophoresis. 2B. Amount of AvBD RT-PCR products relative to that of β-actin products. Data presented are geometric means of four independent experiments ± standard deviation. Values with different letters are significantly different (P < 0.05).