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Table 3 Detection limits of luminescent Salmonella typhimurium.

From: Photonic plasmid stability of transformed Salmonella Typhimurium: A comparison of three unique plasmids

Item Bacterial concentration (CFU) Photonic emissions (RLU/s)
Black tube format (1ml) upper limit (2 s acquisition time)
Background (used for subtraction of sample) - 39
   pAK1-lux 1.1 × 108 ± 1.0 × 107 7,470 ± 136
   pCGLS-1 6.2 × 107 ± 1.2 × 107 6,168 ± 167
   pXEN-1 1.0 × 108 ± 1.0 × 107 7,016 ± 136
Black tube format (1. 0 ml) lower limit (2 s acquisition time)
Background (used for subtraction of sample) - 33
   pAK1-lux 2.7 × 106 ± 1.0 × 107 278 ± 136
   pCGLS-1 1.8 × 106 ± 1.0 × 107 327 ± 136
   pXEN-1 5.1 × 106 ± 1.0 × 107 148 ± 141
Item Bacterial concentration (CFU) Photonic emissions (RLU/s)
96-well black plate format (100 μl) lower limit (30 s acquisition time)
Background (used for subtraction of sample) - 6
   pAK1-lux 3.8 × 103 ± 2.8 × 103 2.0 ± 1.3
   pCGLS-1 2.9 × 103 ± 2.8 × 103 1.1 ± 1.3
   pXEN-1 2.8 × 103 ± 2.7 × 103 1.1 ± 1.2
  1. Luminescent Salmonella typhimurium with three different plasmids (pAK1-lux, pXEN-1, and pCGLS-1); upper and lower detection limits for black tube format (2 s acquisition time) and low detection limits for black 96-well plate format (30 s acquisition time). The results below are bacterial concentration means ± standard error of the mean and photonic emissions means ± standard error of the mean.