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Table 5 Kinetic parameters of Arthrobacter sp. 32c β-D-galactosidase.

From: A new cold-adapted β-D-galactosidase from the Antarctic Arthrobacter sp. 32c – gene cloning, overexpression, purification and properties

Substrate Temperature [°C] Km [mM] kcat [s-1] kcat/Km [s-1mM-1]
ONPG 10 5.75 ± 0.34 52.4 ± 0.72 9.12 ± 0.71
  20 4.86 ± 0.37 81.0 ± 1.03 16.67 ± 1,60
  30 3.46 ± 0.29 123.9 ± 1.21 35.81 ± 3.66
  40 3.15 ± 0.27 169.9 ± 1.44 53.92 ± 5.56
  50 2.62 ± 0.21 212.4 ± 1.67 81.07 ± 7.76
  55 5.11 ± 0.32 71.2 ± 0.98 13.93 ± 1.14
lactose 10 77.54 ± 1.77 1.76 ± 0.11 0.023 ± 0.002
  20 67.82 ± 1.74 2.36 ± 0.14 0.035 ± 0.003
  30 52.67 ± 1.71 4.81 ± 0.22 0.091 ± 0.007
  40 44.31 ± 1.73 5.73 ± 0.21 0.129 ± 0.010
  50 39.73 ± 1.72 6.98 ± 0.23 0.176 ± 0.014