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Table 1 Purification of recombinant Arthrobacter sp. 32c β-D-galactosidase.

From: A new cold-adapted β-D-galactosidase from the Antarctic Arthrobacter sp. 32c – gene cloning, overexpression, purification and properties

Purification step Volume (ml) Protein (mg) Specific activity (U mg-1) Total activity (U) Purification (fold) Recovery (%)
E. coli LMG plysN pBADMyc-HisA-32cβ-gal
Cell extract 30 580 13.8 8004 1.0 100
Affinity chromatography 3.2 27 155.9 4209 21.0 53
P. pastoris GS115 pPICZαA-32cβ-gal
Broth 1000 3400 28.7 97580 1.0 100
Protein precipitation 54 340 136.1 46274 10.0 47
Affinity chromatography 11 137 154.7 21194 24.8 22
P. pastoris GS115 pGAPZαA-32cβ-gal
Broth 1000 5200 16.2 84240 1.0 100
Protein precipitation 46 450 102.7 46215 11.6 55
Affinity chromatography 10 97 153.1 14851 53.6 18