Table 1 Purification of recombinant Arthrobacter sp. 32c β-D-galactosidase.
Purification step | Volume (ml) | Protein (mg) | Specific activity (U mg-1) | Total activity (U) | Purification (fold) | Recovery (%) |
|---|---|---|---|---|---|---|
E. coli LMG plysN pBADMyc-HisA-32cβ-gal | ||||||
Cell extract | 30 | 580 | 13.8 | 8004 | 1.0 | 100 |
Affinity chromatography | 3.2 | 27 | 155.9 | 4209 | 21.0 | 53 |
P. pastoris GS115 pPICZαA-32cβ-gal | ||||||
Broth | 1000 | 3400 | 28.7 | 97580 | 1.0 | 100 |
Protein precipitation | 54 | 340 | 136.1 | 46274 | 10.0 | 47 |
Affinity chromatography | 11 | 137 | 154.7 | 21194 | 24.8 | 22 |
P. pastoris GS115 pGAPZαA-32cβ-gal | ||||||
Broth | 1000 | 5200 | 16.2 | 84240 | 1.0 | 100 |
Protein precipitation | 46 | 450 | 102.7 | 46215 | 11.6 | 55 |
Affinity chromatography | 10 | 97 | 153.1 | 14851 | 53.6 | 18 |