Table 2 Oligonucleotide primers used in this study.
From: A novel DNA-binding protein modulating methicillin resistance in Staphylococcus aureus
Primer name | Nucleotide sequence (5'-3')a | Reference |
|---|---|---|
Markerless deletion construction | ||
me62attB1 | GGGGACAAGTTTGTACAAAAAAGCAGGCTCCACTGGCTTATTCGCTTGA | This study |
me51BamHI | ATTAGGATCC TTAGTACATATCTAGGCCTA | This study |
me62BamHI | ATTAGGATCC ACTCTGTCTATCCATTCTGT | This study |
me62attB2 | GGGGACCACTTTGTACAAGAAAGCTGGGTTGTGCGACAAGGATTGCGA | This study |
Cloning | ||
me94BamHI | ATTAGGATCC TCTTCAATCACTTGGCCAAT | This study |
me94Asp718 | ATTAGGTACC AAGGTGCTGATGGTTATGAA | This study |
me65BamHI | ATTAGGATCC GATAGACAGAGTTTTACAGA | This study |
me65XhoI | ATTACTCGAG GATATGTACTAATTCTTCTT | This study |
Protein-DNA binding and EMSA | ||
me36F | TGATAACACCTTCTACACCT | This study |
me36Rbiot | BIOT-AACCCGACAACTACAACTAT | This study |
me36R | AACCCGACAACTACAACTAT | This study |
Primer extension | ||
me97 | BIOT-ACTCTGTCTATCCATTCTGT | This study |
me98 | BIOT-CAGCCTCTATACGAACCATT | This study |
me52F | CCACTGGCTTATTCGCTTGA | This study |
me52R | TGTGCGACAAGGATTGCGAT | This study |
Gene/transcript detection | ||
mecAP4 | TCCAGATTACAACTTCACCAGG | [42] |
mecAP7 | CCACTTCATATCTTGTAACG | [42] |
SA1665F | TTCGTATAGAGGCTGGTTAG | This study |
SA1665R | AATTGGTTGGTTATCTGGAT | This study |
mecR1F | TGACACGACTTCTTCGGTTA | This study |
mecR1R | AACGTATATGTTCATGGCGA | This study |
SA1664F | TCAGCATGTAGATAACGCAA | This study |
SA1664R | ATGTCACAATTGTTCTTGCT | This study |
SA1666F | GACCATTATATTGTGCGACA | This study |
SA1666R | TTGTGCCTTAGGATGTATCA | This study |
SA1667F | TTGTGCCTTAGGATGTATCA | This study |
SA1667R | TAATACCGTGTGATGAAGCT | This study |