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Figure 5 | BMC Microbiology

Figure 5

From: A novel DNA-binding protein modulating methicillin resistance in Staphylococcus aureus

Figure 5

Northern and Western blot analyses. A, Transcription of SA1665 over growth in CHE482, with RNA harvested at the OD600 nm values indicated. B, Transcription of SA1665 from CHE482 grown to OD600 nm 0.25 and either left uninduced (-) or induced with either 4 or 120 μg/ml of cefoxitin fo 0', 10' and 30'. C, Transcriptional profiles of SA1664, SA1665, SA1666 and SA1667 in CHE482 and ΔCHE482, grown to OD600 nm 0.25 and either uninduced or induced with cefoxitin 4 μg/ml for 0', 10' or 30'. Approximate sizes of transcripts, in kb, are indicated on the right of the blots. D, Transcription of mecA and mecR1 in CHE482 and ΔCHE482, grown to OD 0.25 and either left uninduced or induced with cefoxitin (4 μg/ml) and sampled after 0', 10' and 30'. Ethidium bromide stained 16S rRNA bands from all Northern gels are shown as a comparative indication of RNA loading. E, Western blots showing amounts of PBP2a in ZH44 and ZH73 and their respective SA1665 deletion mutants, before (0') and after induction with 4 μg/ml of cefoxitin for 10' and 30'.

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