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Table 2 Selected proteins with altered abundance under nitrogen limitation.

From: Quantitative proteomics of nutrient limitation in the hydrogenotrophic methanogen Methanococcus maripaludis

ORF # Function Average log2 ratioa
  Nitrogen fixation  
MMP0853 NifH, nitrogenase reductase 2.29 ± 0.16
MMP0854 NifI1 1.68 ± 0.57
MMP0855 NifI2 2.10 ± 0.23
MMP0856 NifD, nitrogenase 2.45 ± 0.15
MMP0857 NifK, nitrogenase 2.03 ± 0.22 (7.09)b
MMP0858 NifE 1.85 ± 0.42
MMP0859 NifN 1.65 ± 0.30
MMP0860 NifX 3.13 ± 0.60
MMP0446 NifX-NifB superfamily 1.05 ± 0.40
  Ammonia transport and regulation  
MMP0064 GlnK1 1.30
MMP0065 AmtB1 2.81 ± 0.31
MMP0066 GlnB 0.45 ± 0.41
MMP0067 GlnK2 1.59 ± 0.48
  Ammonia assimilation  
MMP1206 GlnA, glutamine synthetase 1.23
  Molybdate transport  
MMP0205 ModA, molybdate binding protein 2.11 ± 0.47
MMP0507 ModA, molybdate binding protein 2.24 ± 0.50
MMP0516 ModD, molybdate transporter subunit 0.73 ± 0.23
  1. aAverage of four log2 ratios: 14N-labeled nitrogen-limited compared with 15N-labeled H2-limited, 15N-labeled nitrogen-limited compared with 14N-labeled H2-limited, 15N-labeled nitrogen-limited compared with 14N-labeled phosphate-limited, and 14N-labeled nitrogen-limited compared with 15N-labeled phosphate limited. Standard deviations are given. Where protein abundance was affected by a second nutrient limitation, average is from two ratios only, each nitrogen-limited compared with the non-affecting nutrient limitation.
  2. bValue in parentheses represents measurements of mRNA by qRT-PCR.