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Table 2 Selected proteins with altered abundance under nitrogen limitation.

From: Quantitative proteomics of nutrient limitation in the hydrogenotrophic methanogen Methanococcus maripaludis

ORF #

Function

Average log2 ratioa

 

Nitrogen fixation

 

MMP0853

NifH, nitrogenase reductase

2.29 ± 0.16

MMP0854

NifI1

1.68 ± 0.57

MMP0855

NifI2

2.10 ± 0.23

MMP0856

NifD, nitrogenase

2.45 ± 0.15

MMP0857

NifK, nitrogenase

2.03 ± 0.22 (7.09)b

MMP0858

NifE

1.85 ± 0.42

MMP0859

NifN

1.65 ± 0.30

MMP0860

NifX

3.13 ± 0.60

MMP0446

NifX-NifB superfamily

1.05 ± 0.40

 

Ammonia transport and regulation

 

MMP0064

GlnK1

1.30

MMP0065

AmtB1

2.81 ± 0.31

MMP0066

GlnB

0.45 ± 0.41

MMP0067

GlnK2

1.59 ± 0.48

 

Ammonia assimilation

 

MMP1206

GlnA, glutamine synthetase

1.23

 

Molybdate transport

 

MMP0205

ModA, molybdate binding protein

2.11 ± 0.47

MMP0507

ModA, molybdate binding protein

2.24 ± 0.50

MMP0516

ModD, molybdate transporter subunit

0.73 ± 0.23

  1. aAverage of four log2 ratios: 14N-labeled nitrogen-limited compared with 15N-labeled H2-limited, 15N-labeled nitrogen-limited compared with 14N-labeled H2-limited, 15N-labeled nitrogen-limited compared with 14N-labeled phosphate-limited, and 14N-labeled nitrogen-limited compared with 15N-labeled phosphate limited. Standard deviations are given. Where protein abundance was affected by a second nutrient limitation, average is from two ratios only, each nitrogen-limited compared with the non-affecting nutrient limitation.
  2. bValue in parentheses represents measurements of mRNA by qRT-PCR.