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Table 1 Selected proteins with altered abundance under H2 limitation.

From: Quantitative proteomics of nutrient limitation in the hydrogenotrophic methanogen Methanococcus maripaludis

ORF # Function Average log2 ratioa
MMP0820 FrcA, coenzyme F420-reducing hydrogenase 1.30 ± 0.56
MMP1382 FruA, coenzyme F420-reducing hydrogenase 0.77 ± 0.16
MMP1384 FruG, coenzyme F420-reducing hydrogenase 0.88
MMP1385 FruB, coenzyme F420-reducing hydrogenase 0.90
MMP1460 EhaM, energy-conserving hydrogenase A 0.56 ± 0.08
MMP1463 EhaP, energy-conserving hydrogenase A polyferredoxin subunit 0.64 ± 0.27
MMP0058 Mer, methylenetetrahydromethanopterin reductase 0.58
MMP1245 FwdF, formylmethanofuran dehydrogenase 0.20
MMP1247 FwdD, formylmethanofuran dehydrogenase 0.23
MMP1248 FwdA, formylmethanofuran dehydrogenase 0.27
MMP1249 FwdC, formylmethanofuran dehydrogenase 0.28 ± 0.07
MMP1697 HdrA, heterodisulfide reductase 0.35 ± 0.18
MMP1696 VhuD, F420 non-reducing hydrogenase 0.35 ± 0.11
MMP1695 VhuG, F420 non-reducing hydrogenase 0.34
MMP1694 VhuA, F420 non-reducing hydrogenase 0.29
MMP0372 Mtd, F420-dependent methylenetetrahydromethanopterin dehydrogenase 0.35 ± 0.10 (0.89)b
MMP1054 HdrC2, heterodisulfide reductase 0.33
MMP1053 HdrB2, heterodisulfide reductase 0.33 ± 0.11
MMP1563 MtrB, methyltransferase 0.27 ± 0.16
MMP1564 MtrA, methyltransferase 0.09
MMP0127 Hmd, H2-dependent methylenetetrahydromethanopterin dehydrogenase -2.08 (-3.57)b
MMP0125 Hypothetical protein -1.19
MMP0875 S-layer protein -1.25
MMP1176 Putative iron transporter subunit -0.83
MMP1206 GlnA, glutamine synthetase -0.35
  1. aAverage of four log2 ratios: 15N-labeled H2-limited compared with 14N-labeled nitrogen-limited, 14N-labeled H2-limited compared with 15N-labeled nitrogen-limited, 15N-labeled H2-limited compared with 14N-labeled phosphate-limited, and 14N-labeled H2-limited compared with 15N-labeled phosphate limited. Standard deviations are given. Where protein abundance was affected by a second nutrient limitation (other than H2), the average is from two ratios only, each H2-limited compared with the non-affecting nutrient limitation.
  2. bValues in parentheses represent measurements of mRNA by qRT-PCR.