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Table 2 Nitrogen-fixing ability of marked rhizobial strains.

From: Assessing the suitability of antibiotic resistance markers and the indirect ELISA technique for studying the competitive ability of selected Cyclopia Vent. rhizobia under glasshouse and field conditions in South Africa

Treatment

Total dry weight (mg)

Nodule biomass (mg)

Nodule number

Uninoculated

0.06 ± 0.04 a

0.00 ± 0.00 a

0.0 ± 0.0 a

Inoculated

0.72 ± 0.01 b

33.33 ± 0.07 b

19.6 ± 0.1 b

t (1,83)

2.58 **

2.60 **

3.49 **

PPRICI3 Parent

0.87 ± 0.13

18.60 ± 0.64

14.8 ± 0.5

PPRICI3Mkd1

0.70 ± 0.14

23.60 ± 0.78

13.2 ± 0.7

PPRICI3Mkd2

0.68 ± 0.10

15.40 ± 0.48

11.2 ± 0.5

PPRICI3Mkd3

1.26 ± 0.13

18.00 ± 0.62

12.6 ± 0.5

F (3,16)

2.06 ns

0.51 ns

0.17 ns

UCT40a Parent

2.26 ± 0.19 a

75.76 ± 1.36 a

20.0 ± 0.7 a

UCT40aMkd1

1.83 ± 0.23 a

74.70 ± 1.38 a

24.3 ± 0.7 a

UCT40aMkd2

2.13 ± 0.20 a

81.94 ± 1.20 a

31.6 ± 0.7 a

UCT40aMkd3

0.12 ± 0.06 b

0.00 ± 0.00 b

0.0 ± 0.0 b

F (3,16)

4.35 *

10.30 **

8.13 **

UCT44b Parent

0.37 ± 0.13

31.25 ± 0.43

18.0 ± 0.4

UCT44bMkd1

0.90 ± 0.12

56.00 ± 0.81

33.4 ± 0.8

UCT44bMkd2

0.51 ± 0.09

23.20 ± 0.47

18.4 ± 0.5

UCT44bMkd3

0.66 ± 0.12

25.60 ± 0.60

18.2 ± 0.6

F (3,16)

1.61 ns

2.22 ns

2.94 ns

UCT61a Parent

0.84 ± 0.12

39.82 ± 0.93

25.4 ± 0.7

UCT61aMkd1

0.54 ± 0.09

22.64 ± 0.44

16.0 ± 0.5

UCT61aMkd2

0.61 ± 0.10

34.02 ± 0.73

21.6 ± 0.5

UCT61aMkd3

1.07 ± 0.14

48.10 ± 1.04

32.0 ± 0.8

F (3,16)

2.79 ns

1.63 ns

1.79 ns

  1. Values are mean ± SE (n = 5) and different letters within a column indicate significant differences. ** Denotes a significant effect at P < 0.01, * = P < 0.05 and ns = no significant effect.