Table 2 PCR primers used in this study

WAB1

WAB2

5'-GAGAATTC ACCACATGGAACATCTGCTG-3'

5'-GAAGATCT GGGAGCACGCAACTGAG-3'

Forward and reverse primers for amplifying 1.4 kbp upstream arm used in knockout of sgcR3

WCD1

WCD2

5'-AAGATCT CTGATCGGCAAGTACGGAGAC-3'

5'-AAAGCTT GGTGACAGTTCCGAGGAGGTT-3'

Forward and reverse primers for amplifying 1.4 kbp downstream arm used in knockout of sgcR3

H1

H2

5'-CAGAATTC TCTCCTACGCCCTGCTCATC-3'

5'-ACTCTAGA AGAACCGCTCGGCCTGTC-3'

Forward and reverse primers for amplifying 2,539 bp sgcR3 including its native promoter

sgcR3_FOR

sgcR3_REV

5'-GCATATG GCGGCACACGACGTGAG-3'

5'-AGGATCC CAGCTCCCCTCCTGGGAG-3'

Forward and reverse primers for amplifying 1,188 bp sgcR3 coding region

P17

P18

5'-ATGGATCC GCCCCTCGCTCATTGTCG-3'

5'-ATGAATTC CGGATCGGATGTGCTGGTCT-3'

Forward and reverse primers for amplifying 2,461 bp sgcR1R2 including its native promoter

hrdBRT1

hrdBRT2

5'-TGGTCGAGGTCATCAACAAG-3'

5'-TGGACCTCGATGACCTTCTC-3'

Forward and reverse primers used for the detection of hrdB transcripts

R1RT1

R1RT2

5'-GAAAAGTGACTCTGCCCAACGC-3'

5'-CGCTGCACATGGGAACGATC-3'

Forward and reverse primers used for the detection of sgcR1 transcripts

R2RT1

R2RT2

5'-ACCACGAACACCATCGAGGAC-3'

5'-CGGAAGATGCGGGTGAAATG-3'

Forward and reverse primers used for the detection of sgcR2 transcripts

R3RT1

R3RT2

5'-GTGGGCGAACGGGAGACGGT-3'

5'-TGCCGAGGGCCGATCAGAGG-3'

Forward and reverse primers used for the detection of sgcR3 transcripts

A1RT1

A1RT2

5'-CAGAACATATTCAAGGACTGGC-3'

5'-CACTTGTACTGTCGGGTGGTT-3'

Forward and reverse primers used for the detection of sgcA1 transcripts

C4RT1

C4RT2

5'-CTGTGGCTACGGACGAGATTG-3'

5'-CCGAACAGAACATCCCCATCT-3'

Forward and reverse primers used for the detection of sgcC4 transcripts

sgcA1p_FOR_F

sgcA1p_REV

5'-CAGCTGGAGTACGCGATGG-3'

5'-CGACAATGGCGACCTC TTTG-3'

Forward and reverse primers for amplifying 438 bp upstream of sgcA1

sgcB1p_FOR_F

sgcB1p_REV

5'-GAGGGGTTTTGAGGGGTGAA-3'

5'-GATAGGGGTCGGGGGACATC-3'

Forward and reverse primers for amplifying 496 bp upstream of sgcB1

sgcC1p_FOR_F

sgcC1p_REV

5'-GGTGCAACACCGCAGATCC-3'

5'-GAGCTGCGCGGAAGGAAG-3'

Forward and reverse primers for amplifying 481 bp upstream of sgcC1

sgcD2p_FOR_F

sgcD2p_REV

5'-CCTGGAACCATCCGCGAAAC-3'

5'-CGGTCGATCACCAGCACTTC-3'

Forward and reverse primers for amplifying 448 bp upstream of sgcD2

sgcKp_FOR_F

sgcKp_REV

5'-AGGATCATCGTCCCGTCTC-3'

5'-GAGCCAGTCGAACGACTCCA-3'

Forward and reverse primers for amplifying 471 bp upstream of sgcK

R1R2p_FOR_F

R1R2p_REV

5'-ACGATCGTGTCCGCGTAGG-3'

5'-AACGATCGATTCCGTTCAGG-3'

Forward and reverse primers for amplifying 455 bp upstream of sgcR1R2

R3p_FOR_F

R3p_REV

5'-GCCCACGCCCTCTGATCG-3'

5'-CGGGACCATGCTCCGTGTA-3'

Forward and reverse primers for amplifying 467 bp upstream of sgcR3

cagApEMSA1

cagApEMSA2

5'-CACGCTCTGTCCGTCACTCA-3'

5'-ATCCGCTCCCGAAGGTGG-3'

Forward and reverse primers for amplifying 429 bp upstream of cagA