Table 1 Bacterial strains and plasmids used in this study

Strains

Escherichia coli DH5α

General cloning host

[36]

E. coli ET12567/pUZ8002

Strain used for E. coli/Streptomyces conjugation

[34]

E. coli BL21 (DE3)

Strain used for the expression of SgcR3 protein

Novagen

Bacillus subtilis CMCC(B) 63501

Strain used for C-1027 bioassays

[35]

Streptomyces globisporus C-1027

Wild type C-1027 producing strain

[1]

S. globisporus R3KO

S. globisporus C-1027 with disruption of sgcR3, Th^r

This work

Plasmids

pUC18

General cloning vector, Ap^r

[36]

pET-16b

E. coli expression vector, Ap^r

Novagen

pKC1139

E. coli/Streptomyces shuttle vector, Am^r

[30]

pOJ260

Suicide vector nonreplicating in Streptomyces, Am^r

[30]

pSET152

Streptomyces integrative vector, Am^r

[30]

pL646

pSET152 derivative plasmid containing ermE* p and the ribosome-binding site of the tuf1 gene upstream atrAc gene, Am^r

[37]

pOJR3KO

pOJ260 derivative plasmid with the disruption of sgcR3, Th^r

This work

pKCR3

pKC1139 derivative plasmid containing 2,539 bp fragment of sgcR3 including its native promoter, Am^r

This work

pSETR3

pSET152 derivative plasmid containing 2,539 bp fragment of sgcR3 including its native promoter, Am^r

This work

pLR3

pL646 derivative plasmid containing 1,188 bp coding region of sgcR3 instead of atrAc, Am^r

This work

pKCR1R2

pKC1139 derivative plasmid containing 2,461 bp fragment of sgcR1R2 including its native promoter, Am^r

This work

pKCER1R2

pKC1139 derivative plasmid containing 2,461 bp fragment of sgcR1R2 under the ermE* p, Am^r

This work