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Table 1 Bacterial strains and plasmids used in this study

From: Role of sgcR3 in positive regulation of enediyne antibiotic C-1027 production of Streptomyces globisporus C-1027

Strains or plasmids Description* Reference
Strains   
Escherichia coli DH5α General cloning host [36]
E. coli ET12567/pUZ8002 Strain used for E. coli/Streptomyces conjugation [34]
E. coli BL21 (DE3) Strain used for the expression of SgcR3 protein Novagen
Bacillus subtilis CMCC(B) 63501 Strain used for C-1027 bioassays [35]
Streptomyces globisporus C-1027 Wild type C-1027 producing strain [1]
S. globisporus R3KO S. globisporus C-1027 with disruption of sgcR3, Thr This work
Plasmids   
pUC18 General cloning vector, Apr [36]
pET-16b E. coli expression vector, Apr Novagen
pKC1139 E. coli/Streptomyces shuttle vector, Amr [30]
pOJ260 Suicide vector nonreplicating in Streptomyces, Amr [30]
pSET152 Streptomyces integrative vector, Amr [30]
pL646 pSET152 derivative plasmid containing ermE* p and the ribosome-binding site of the tuf1 gene upstream atrAc gene, Amr [37]
pOJR3KO pOJ260 derivative plasmid with the disruption of sgcR3, Thr This work
pKCR3 pKC1139 derivative plasmid containing 2,539 bp fragment of sgcR3 including its native promoter, Amr This work
pSETR3 pSET152 derivative plasmid containing 2,539 bp fragment of sgcR3 including its native promoter, Amr This work
pLR3 pL646 derivative plasmid containing 1,188 bp coding region of sgcR3 instead of atrAc, Amr This work
pKCR1R2 pKC1139 derivative plasmid containing 2,461 bp fragment of sgcR1R2 including its native promoter, Amr This work
pKCER1R2 pKC1139 derivative plasmid containing 2,461 bp fragment of sgcR1R2 under the ermE* p, Amr This work
  1. *Abbreviations: Apr, ampicillin resistance; Amr, apramycin resistance; Thr, thiostrepton resistance.