|
Human MDM
|
|---|
| |
Viable
|
UV-Inactivated
|
PBS
|
|---|
|
IL-1β
|
31 ± 6.1*
|
33 ± 1.4*
|
0.7 ± 0.04
|
|
IL-6
|
385 ± 13.8*
|
439 ± 4.0*
|
3.2 ± 0.1
|
|
IL-8
|
5784 ± 149*
|
5368 ± 564*
|
116 ± 7.8
|
|
G-CSF
|
63.1 ± 5.5*
|
72 ± 2.4*
|
6.2 ± 0.1
|
|
IFN-γ
|
270 ± 24*
|
339 ± 3.9*
|
9 ± 3.6
|
|
MCP-1
|
298 ± 9.3*
|
318 ± 8.3*
|
36 ± 3.9
|
|
MIP-1α
|
1056 ± 16*
|
1068 ± 4.0*
|
176 ± 10.9
|
|
MIP-1β
|
2514 ± 57*
|
2403 ± 19*
|
810 ± 47
|
|
RANTES
|
66 ± 1.5*
|
74 ± 9.9*
|
11.4 ± 0.4
|
|
TNF-α
|
7456 ± 334*
|
8616 ± 697*
|
20 ± 2.0
|
- aHuman MDM were inoculated with M. genitalium G37 (MOI 10) or an equal volume of the PBS vehicle as a control into triplicate wells. Culture supernatants were collected 6 h PI to quantify secreted cytokines as described in the Methods. Values are expressed as the mean ± SEM pg/mL supernatant. PBS values are presented to indicate basal cytokine elaboration. Data collected following exposure to M. genitalium strain M2300 were similar in pattern and magnitude. ND, not detected. *, p < 0.01 using ANOVA.
