Human MDM |
---|
 | Viable | UV-Inactivated | PBS |
---|
IL-1β
| 31 ± 6.1* | 33 ± 1.4* | 0.7 ± 0.04 |
IL-6
| 385 ± 13.8* | 439 ± 4.0* | 3.2 ± 0.1 |
IL-8
| 5784 ± 149* | 5368 ± 564* | 116 ± 7.8 |
G-CSF
| 63.1 ± 5.5* | 72 ± 2.4* | 6.2 ± 0.1 |
IFN-γ
| 270 ± 24* | 339 ± 3.9* | 9 ± 3.6 |
MCP-1
| 298 ± 9.3* | 318 ± 8.3* | 36 ± 3.9 |
MIP-1α
| 1056 ± 16* | 1068 ± 4.0* | 176 ± 10.9 |
MIP-1β
| 2514 ± 57* | 2403 ± 19* | 810 ± 47 |
RANTES
| 66 ± 1.5* | 74 ± 9.9* | 11.4 ± 0.4 |
TNF-α
| 7456 ± 334* | 8616 ± 697* | 20 ± 2.0 |
- aHuman MDM were inoculated with M. genitalium G37 (MOI 10) or an equal volume of the PBS vehicle as a control into triplicate wells. Culture supernatants were collected 6 h PI to quantify secreted cytokines as described in the Methods. Values are expressed as the mean ± SEM pg/mL supernatant. PBS values are presented to indicate basal cytokine elaboration. Data collected following exposure to M. genitalium strain M2300 were similar in pattern and magnitude. ND, not detected. *, p < 0.01 using ANOVA.