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Table 2 Cytokine elaboration from human monocyte-derived macrophages following exposure to M. genitalium G37a.

From: Intracellular Mycoplasma genitalium infection of human vaginal and cervical epithelial cells elicits distinct patterns of inflammatory cytokine secretion and provides a possible survival niche against macrophage-mediated killing

Human MDM

 

Viable

UV-Inactivated

PBS

IL-1β

31 ± 6.1*

33 ± 1.4*

0.7 ± 0.04

IL-6

385 ± 13.8*

439 ± 4.0*

3.2 ± 0.1

IL-8

5784 ± 149*

5368 ± 564*

116 ± 7.8

G-CSF

63.1 ± 5.5*

72 ± 2.4*

6.2 ± 0.1

IFN-γ

270 ± 24*

339 ± 3.9*

9 ± 3.6

MCP-1

298 ± 9.3*

318 ± 8.3*

36 ± 3.9

MIP-1α

1056 ± 16*

1068 ± 4.0*

176 ± 10.9

MIP-1β

2514 ± 57*

2403 ± 19*

810 ± 47

RANTES

66 ± 1.5*

74 ± 9.9*

11.4 ± 0.4

TNF-α

7456 ± 334*

8616 ± 697*

20 ± 2.0

  1. aHuman MDM were inoculated with M. genitalium G37 (MOI 10) or an equal volume of the PBS vehicle as a control into triplicate wells. Culture supernatants were collected 6 h PI to quantify secreted cytokines as described in the Methods. Values are expressed as the mean ± SEM pg/mL supernatant. PBS values are presented to indicate basal cytokine elaboration. Data collected following exposure to M. genitalium strain M2300 were similar in pattern and magnitude. ND, not detected. *, p < 0.01 using ANOVA.