aHuman vaginal (n = 3 donors), ectocervical or endocervical ECs were inoculated with M. genitalium G37 (MOI 10). An equal volume of the PBS vehicle was added and processed in parallel as a control. Culture supernatants were collected 48 h PI to quantify secreted cytokines. Values are expressed as the mean ± SEM pg/mL from triplicate wells. Cytokine elaboration pattern and magnitudes induced following exposure to strain M2300 were not significantly different than G37. PBS values are presented to indicate basal cytokine elaboration from each cell type. ND, not detected. *, p < 0.05 vs. PBS control using Student's t-test.