Figure 7From: Yersinia outer protein YopE affects the actin cytoskeleton in Dictyostelium discoideumthrough targeting of multiple Rho family GTPasesYopE blocks the effects of RacH on growth and endocytosis. (A) Growth in nutrient medium. Cultures were inoculated at a density of 0.5 × 106 cells/ml. The graph is representative of two independent experiments, each run in duplicate. * P < 0.05 of GFP-RacH relative to AX2, †P < 0.05 of GFP-RacH/myc-YopE relative to AX2; ANOVA. (B) Fluid-phase endocytosis of FITC-dextran. Cells were resuspended in fresh axenic medium at 5 × 106 cells/ml in the presence of 2 mg/ml FITC-dextran. Fluorescence from the internalized marker was measured at selected time points. Data are presented as relative fluorescence, AX2 being considered 100%. Four independent experiments are averaged. For clarity, error bars are depicted only in one direction. * P < 0.05 relative to AX2, ANOVA.Back to article page