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Figure 4 | BMC Microbiology

Figure 4

From: DNA-binding by Haemophilus influenzae and Escherichia coli YbaB, members of a widely-distributed bacterial protein family

Figure 4

Analysis of stoichiometries and affinities of YbaB Ec and YbaB Hi binding to b-WT DNA. Data from the experiments shown in Fig. 3. (A) Binding of YbaBEc. Symbols: (black circle), first binding step; (black square), second binding step. The lines are least-squares fits to Eqs 4 and 5, returning stoichiometry values of 1.93 ± 0.14 for the first binding step and 2.16 ± 0.14 for the second. From the logarithm of the free protein concentration at the midpoint of each binding transition we estimate that Ka,1 = 1.7 ± 0.8 × 1014 M-2 and Ka,2 = 2.9 ± 0.5 × 1013 M-2. The ranges given for these parameters are 95% confidence limits calculated for the least squares fits. (B) Binding of YbaBHi. Symbols: (black circle), first binding step; (black square), second binding step. The lines are least-squares fits to Eqs 4 and 5, returning stoichiometry values of 2.09 ± 0.16 for the first binding step and 2.18 ± 0.19 for the second. From the logarithm of the free protein concentration at the midpoint of each binding transition we estimate Ka,1 = 1.7 ± 0.7 × 1013 M-2 and Ka,2 = 3.0 ± 1.4 × 1012 M-2. The ranges given for these parameters are 95% confidence limits calculated for the least squares fits.

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