Figure 2

Nucleotide sequences (5' to 3') of DNA probes used for EMSA in these studies, based on the operator 2 sequences of B. burgdorferi erpAB[7, 8, 10]. Underlined nucleotides identify the wild-type (GTnAC) and mutated sequences to which B. burgdorferi EbfC will either bind or not bind, respectively (see Fig. 5). Mutated nucleotides are indicated by lower case letters. All probes used in EMSAs were labeled with a biotin moiety at the one 5' end.