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Figure 5 | BMC Microbiology

Figure 5

From: Discovery of novel inhibitors of Streptococcus pneumoniae based on the virtual screening with the homology-modeled structure of histidine kinase (VicK)

Figure 5

Inhibition ratio of VicK' protein autophosphorylation by six lead compounds with antibacterial effects (from the 23 compounds). The inhibitory activities of the compounds for the ATPase activity of the VicK' protein was measured using the Kinase-Glo™ Luminescent Kinase Assay. Briefly, purified VicK' protein(6 μg/50 μl) was pre-incubated with compounds(final concentration, 200 μM) in a reaction buffer containing 40 mM Tris-HCl (pH 7.5), 20 mM MgCl2 and 0.1 mg/ml BSA, at room temperature for 10 min. Then ATP (5 μM) was added for another incubation of 10 min at room temperature, and detected the rest amount of ATP.

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BMC Microbiology

ISSN: 1471-2180

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