Figure 3From: Discovery of novel inhibitors of Streptococcus pneumoniae based on the virtual screening with the homology-modeled structure of histidine kinase (VicK)(A) SDS-PAGE analysis of VicK' purification (B) Identification of kinase activity of VicK' protein in vitro. Variant amounts of VicK' proteins were added into reaction systems containing a constant ATP concentration (5 μM). Each assay was performed in quadruplicate and repeated three times. Luminescent output is inversely correlated with the concentration of the kinase.Back to article page