Figure 2

PpoR binds 3-oxo-C6-HSL. E. coli M15 (pRep4) containing either pQEPpoR or pQE30 were grown in LB in the presence of various AHLs (1 μM) added separately and protein expression induced with IPTG (1 μM). After 3.5 hours of growth post induction, AHLs were extracted from the cell pellets and visualized by TLC overlaid with A. tumefaciens NTL4 (pZLR4). The standards used are synthetic AHLs. In the figure, the lanes are marked as follows; S – AHL standards, 1 – AHL extracted from E. coli/pQE30 cell pellets grown with 3-oxo-C6-HSL supplementation and 2 – AHL extracted from E. coli/pQEPpoR cell pellets grown with 3-oxo-C6-HSL supplementation.