Characterization of α-isopropylmalate synthases containing different copy numbers of tandem repeats in Mycobacterium tuberculosis

Table 1 Kinetic parameters, V_max and K_m, of α-IPMS reacting to α-ketoisovaleric acid and acetyl CoA^a

^a At pH 8.5 and 37°C. The apparent K_m and V_max values were determined by varying the concentrations of one substrate at a fixed saturating concentration of the other substrate. α-ketoisovaleric acid and acetyl CoA was fixed at 2 mM and 0.8 mM, respectively. Data are the average of two assays. Prism software (version 3.08) was used for nonlinear regression, curve fit analysis to calculate K_m and V_max.
^bk_cat= V_max/[E] (μmol s^-1mg^-1)/(mol mg^-1)

ISSN: 1471-2180