Distribution of TcKAP6 in T. cruzi. Immunolocalization of TcKAP6 in epimastigotes (A-D), amastigotes/intermediates forms (E-H) and trypomastigotes (I-L) of T. cruzi. As observed for TcKAP4, this protein was also distributed throughout kDNA disk in epimastigotes (B-inset) and amastigotes (F-arrow and inset), and at the periphery of the kinetoplast in intermediate forms (F-arrowhead) and trypomastigotes (J-inset). (A-E-I) Phase-contrast image, (B-F-J) location of TcKAP6 in the kinetoplasts of T. cruzi, (C-G-K) iodide propidium labeling and (D-H-L) the overlay image. k = kinetoplast, n = nucleus. Bars = 5 μm.