Polymorphism and selection of rpoS in pathogenic Escherichia coli

BMC Microbiology

Table 1 Suc⁺⁺ mutants selected from VTEC strains with attenuated or intact RpoS functions.

Sero-pathotype	Serotype	Strain	Source	Host	Number of mutants		Ratio of rpoS/Suc⁺⁺
					Suc⁺⁺	rpoS
A	O157:H7	EDL933	J. Kaper	Human	12	11	0.92
B	O121:H19	CL106	LFZ	Human	12	10	0.83
	O111:NM	R82F2	LFZ	Human	N/A		N/A
C	O5:NM	N00-4067	BCCDC, NLEP	Human	12	12	1.00
	O113:H21	CL3	LFZ	Human	N/A		N/A
	O121:NM	N99-4390	BCCDC, NLEP	Human	N/A		N/A
D	O103:H25	N00-4859	BCCDC, NLEP	Human	12	12	1.00
	O172:NM	EC6-484	LFZ	Bovine	12	8	0.67
E	O84:NM	EC2-044	LFZ	Bovine	12	12	1.00
	O98:H25	EC3-377	LFZ	Bovine	12	12	1.00

Twelve Suc⁺⁺ mutants from each strain were tested for catalase activity using a plate catalase assay. Mutants impaired in catalase were considered as putative rpoS mutants. Detailed VTEC strain information is described elsewhere [29].

ISSN: 1471-2180