Table 1 Bacterial strains and plasmids used in this study

V. cholerae

N16961

Serogroup O1, El Tor biotype

Our lab store

N169-dtatABC

tatABC deletion mutant from N16961

This study

N16961(pBAD24)

N16961 transformed with vector pBAD24

This study

N169-dtatABC(pBAD24)

N169-dtatABC transformed with pBAD24

This study

N169-dtatABC-cp

N169-dtatABC complemented with pBAD-TatABC

This study

N169-dtatABC-BCcp

N169-dtatABC complemented with pBAD-TatBC

This study

N169-dtatE

tatE deletion mutant from N16961

This study

N169-dtatABCE

tatABC and tatE double deletion mutant from N16961

This study

N169-dtatABCE-BCcp

N169-dtatABCE complemented with pBAD24 carrying tatBC

This study

N169-dtatB

tatB deletion mutant from N16961

This study

N169-dtatC

tatC deletion mutant from N16961

This study

E. coli

SM10 λpir

thi thr leu tonA lacY supE recA::RP4-2-Tc::Mu Km

21

JARV16A (dtatAE)

tatA and tatE double deletion mutant from JARV16A

34

MCMTAA(dtatB)

tatB::Kan mutant from MCMTAA

34

B1LK0A (dtatC)

tatC deletion mutant from B1LK0A

34

DADEA (dtatABCDE)

tatABCD and tatE double deletion mutant from DADEA

34

Plasmids

pCVD442

Suicide vector, ori R6K, Amp^r, sacB

21

pDS132

Suicide vector, ori R6K, from pCVD442, Cm^r, sacB

22

pT1

714 bp EcoR I-Kpn I fragment A of tatA cloned into pUC18

This study

pT2

461 bp Xba I-Pst I fragment B of tatC cloned into pT1

This study

pT3

801 bp fragment of cat cloned into Sma I site of pT2

This study

pCT4

1,976 bp fragment of 'A-cat-B' cloned into Sph I site of pCVD442

This study

pUC18C

intact tatABC and upstream fragment cloned between EcoR I and Sac I site of pUC18

This study

pBAD24

pMB1-derived plasmid, Amp^r, araBAD

23

pTatABC-301

intact tatABC fragment of E. coli cloned into pBAD24

This study

pBAD-TatABC

intact tatABC fragment of N16961 cloned into pBAD24

This study

pBAD-TatBC

tatBC fragment of N16961 cloned into pBAD24

This study

pBAD-TatE

tatE fragment of N16961 cloned into pBAD24

This study