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Table 1 Bacterial strains and plasmids used in this study

From: Pleiotropic effects of the twin-arginine translocation system on biofilm formation, colonization, and virulence in Vibrio cholerae

Strains or plasmids Relevant genotype and/or phenotype Source or reference
V. cholerae   
N16961 Serogroup O1, El Tor biotype Our lab store
N169-dtatABC tatABC deletion mutant from N16961 This study
N16961(pBAD24) N16961 transformed with vector pBAD24 This study
N169-dtatABC(pBAD24) N169-dtatABC transformed with pBAD24 This study
N169-dtatABC-cp N169-dtatABC complemented with pBAD-TatABC This study
N169-dtatABC-BCcp N169-dtatABC complemented with pBAD-TatBC This study
N169-dtatE tatE deletion mutant from N16961 This study
N169-dtatABCE tatABC and tatE double deletion mutant from N16961 This study
N169-dtatABCE-BCcp N169-dtatABCE complemented with pBAD24 carrying tatBC This study
N169-dtatB tatB deletion mutant from N16961 This study
N169-dtatC tatC deletion mutant from N16961 This study
E. coli   
SM10 λpir thi thr leu tonA lacY supE recA::RP4-2-Tc::Mu Km 21
JARV16A (dtatAE) tatA and tatE double deletion mutant from JARV16A 34
MCMTAA(dtatB) tatB::Kan mutant from MCMTAA 34
B1LK0A (dtatC) tatC deletion mutant from B1LK0A 34
DADEA (dtatABCDE) tatABCD and tatE double deletion mutant from DADEA 34
Plasmids   
pCVD442 Suicide vector, ori R6K, Ampr, sacB 21
pDS132 Suicide vector, ori R6K, from pCVD442, Cmr, sacB 22
pT1 714 bp EcoR I-Kpn I fragment A of tatA cloned into pUC18 This study
pT2 461 bp Xba I-Pst I fragment B of tatC cloned into pT1 This study
pT3 801 bp fragment of cat cloned into Sma I site of pT2 This study
pCT4 1,976 bp fragment of 'A-cat-B' cloned into Sph I site of pCVD442 This study
pUC18C intact tatABC and upstream fragment cloned between EcoR I and Sac I site of pUC18 This study
pBAD24 pMB1-derived plasmid, Ampr, araBAD 23
pTatABC-301 intact tatABC fragment of E. coli cloned into pBAD24 This study
pBAD-TatABC intact tatABC fragment of N16961 cloned into pBAD24 This study
pBAD-TatBC tatBC fragment of N16961 cloned into pBAD24 This study
pBAD-TatE tatE fragment of N16961 cloned into pBAD24 This study