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Table 1 Bacterial species and strains used in this study and results of pentaplex PCR.

From: A pentaplex PCR assay for the rapid detection of methicillin-resistant Staphylococcus aureus and Panton-Valentine Leucocidin

No. Reference strains 16S rRNAa fem A mec Ab luk S Internalcontrol
1. S. aureus (ATCC 33591) + + + - +
2. S. aureus (ATCC 33592) + + + - +
3. S. aureus (ATCC 43300) + + + - +
4. S. aureus (ATCC 25923)d + + - + +
5. S. aureus (ATCC 49775) + + - + +
6. S. aureus (ATCC 51153)e + + - - +
7. S. epidermidis (ATCC 14990) + - - - +
8. Staphylococcus haemolyticus (ATCC 29970) + - - - +
9. Staphylococcus saprophyticus (ATCC 13518)d + - - - +
10. CoNS methicillin-resistante + - + - +
11. Streptococcus spp. Group A (ATCC 19615)e - - - - +
12. Streptococcus spp. Group B (ATCC 12401)e - - - - +
13. Streptococcus spp. Group Ge - - - - +
14.Streptococcus spp. Group Fe - - - - +
15. Bacillus subtilis (ATCC 6633)e - - - - +
16.Listeria monocytogenes (ATCC 7644)e - - - - +
17. Enterococcus faecium LMG 16192c - - - - +
18. Enterococcus faecalis (ATCC 29212)e - - - - +
19. Corynebacterium sppe - - - - +
20. Escherichia coli (EHEC)e - - - - +
21. E. coli (EPEC)e - - - - +
22.E. coli (ETEC)e - - - - +
23. Klebsiella pneumoniae (ATCC 10031)e - - - - +
24. Shigella sonnei (ATCC 25931)e - - - - +
25. Shigella flexneri (ATCC 12022)e - - - - +
26. Shigella boydii (ATCC 9207)e - - - - +
27.Proteus mirabilis (ATCC 29245)e - - - - +
28. Salmonella typhie - - - - +
29. Pseudomonas aeruginosa (ATCC 27853)e - - - - +
30.Yersinia enterocolitica (ATCC 23715)e - - - - +
31. Vibrio cholerae (O1 classical)e - - - - +
32. Citrobacter freundii (ATCC 8090)e - - - - +
33.Gardnerella sppe - - - - +
34.Candida albicans (ATCC 10231)e - - - - +
  1. a Staphylococcus genus
  2. b methicillin-resistant genotype
  3. c Reference strains from Belgian Co-ordinated Collections of Micro-organisms (BCCM), Ghent,
  4. Belgium
  5. d Obtained from Institute for Medical Research, Malaysia
  6. e Department of Medical Microbiology and Parasitology, School of Medical Sciences, Universiti
  7. Sains Malaysia.
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BMC Microbiology

ISSN: 1471-2180

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