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Table 2 Bacterial strains and plasmids used in this study

From: Involvement of RNA-binding protein Hfq in the osmotic-response regulation of invE gene expression in Shigella sonnei

Bacterial strains and plasmids Genotypes (references)
E. coli   
   N3431 rne-3071ts, lacZ43, LAM-, relA1, spoT1 (CGSC#6975) [36]
S. sonnei   
   HW383 S. sonnei wild-type strain, (Tcr) [7]
   HW506 S. sonnei HW383 without pSS120 plasmid (Tcr, non invasive) [7]
   MS506 HW506 (Tcs) This study
   MS390 HW383 (Tcs) [13]
   MS1632 MS390ΔinvE [11]
   MS2830 MS390ΔcpxR (cpxR: chromosomal activator of virF gene) [13]
   MS4831 MS390Δhfq [11]
   MS4841 MS390Δhns (non invasive) [11]
   MS5400 MS390Δrne 701–892 ::aphA This study
   MS5512 MS390ΔpinvE::paraBAD [11]
S. flexneri   
   2457T S. flexneri 2a wild-type strain, [49]
   2457O 2457T carrying mutation in virF gene (non-invasive) [50]
   MF4835 2457TΔhfq::aphA [11]
Plasmids   
   pBAD-invE PCR-amplified invE gene was cloned into pBAD24 (Apr) [11]
   pHW848 virF-lacZ translational fusion plasmid (Cmr) [8]
   pJK1142 invE and ipa-mxi-spa (TTSS) genes encoding plasmid (Kmr) [4]
   pJK1143 virF-encoding plasmid (Cmr) [4]
   pJM4320 invE-lacZYA transcriptional fusion in pTH18cs5(Cmr) [13]
   pJM4321 invE-lacZYA translational fusion in pTH18cs5(Cmr) [13]
   pTrc99A IPTG inducible expression plasmid(Apr) [51]
   pTrc-hfq PCR-amplified hfq gene was cloned into pTrc99A(Apr) [11]