Table 2 Oligonucleotide primers
From: Chitobiose utilization in Borrelia burgdorferi is dually regulated by RpoD and RpoS
Primer Name | Sequence (5'→3') |
|---|---|
BB0771 F1 | CTTGCAGGACAAATACAAAGAGGC |
BB0771 R1 | GCAGCTCTTATTAATCCCAAGTTGCC |
BB0450 mutF1 | TTCTCCTCTTGGAACCATTCCGGT |
BB0450 mutR1 | ACCATAACCTACCACGCCCTCAAT |
BB0450 mut confirm F1 | GGTTCCATAATATGTTCTCCCTTTCTCAG |
BB0450 mut confirm R1 | CCCAACGCTCGAATTTAAAGACCC |
5' ErmC seq out | GGCCTTTTCCTGAGCCGATTTCAAAG |
3' ErmC seq out | TTCCTTAAAACATGCAGGAATTGACG |
chbC F | GGGAATTCAGCCCAATTCATGGTTTCC |
chbC R | GGCGGAACAGACTCTGGAAGCTTAAT |
BBB04 5'RACE R1 | GCTACAATTGAAAGCGCAACAACAGG |
Oligo(dT) AP | GACCACGCGTATCGATGTCGACTTTTTTTTTTTTTTTTV |
Oligo(dC) AP | GACCACGCGTATCGATGTCGACCCCCCCCCCCCCCCCC |
BBB04 5'RACE R2 | AGCAGCATCTCCACCGTAAGGTAT |