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Table 1 Strains and Plasmids

From: Chitobiose utilization in Borrelia burgdorferi is dually regulated by RpoD and RpoS

Strain or Plasmid

Genotype and Description

Reference

Strains

  

   B. burgdorferi

  

B31-A

High passage non-infectious wild-type

[38]

A74

CoumR; B31-A rpoS mutant

[38]

WC12

CoumR KanR; A74 complemented with rpoS Bb /pCE320

This study

297 rpoN

EryR; 297 rpoN mutant

[19]

RR22

EryR; B31-A rpoN mutant

This study

   E. coli

  

DH5α

supE 44 F- Δlac U169 (ϕ80lac Z ΔM15) hsdR 17 relA 1 endA 1 gyrA 96 thi-1 relA 1

[40]

Plasmids

  

   rpoS Bb /pCE320

KanR ZeoR; Pnat-rpoS

[17]

   pBB0450.1

AmpR EryR; ermC::rpoN

This study