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Figure 6 | BMC Microbiology

Figure 6

From: Chitobiose utilization in Borrelia burgdorferi is dually regulated by RpoD and RpoS

Figure 6

Determination of the chbC transcriptional start site. The chbC transcriptional start site was determined by 5' RACE analysis. (A) One percent TAE agarose gel of the 5' RACE products. A 1 kb ladder was used as a size standard (lane 1) for comparison of 5' RACE products (lane 2, dA-tailed product; lane 3, dG-tailed product). (B) DNA sequence of the dA-tailed 5' RACE product showing the ambiguous chbC transcriptional start site (enlarged font). (C) DNA sequence of the dG-tailed 5' RACE product showing the chbC transcriptional start site (enlarged font). Sequences were determined using the anti-sense primer BBB04 5' RACE R2.

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