Figure 6From: Chitobiose utilization in Borrelia burgdorferi is dually regulated by RpoD and RpoSDetermination of the chbC transcriptional start site. The chbC transcriptional start site was determined by 5' RACE analysis. (A) One percent TAE agarose gel of the 5' RACE products. A 1 kb ladder was used as a size standard (lane 1) for comparison of 5' RACE products (lane 2, dA-tailed product; lane 3, dG-tailed product). (B) DNA sequence of the dA-tailed 5' RACE product showing the ambiguous chbC transcriptional start site (enlarged font). (C) DNA sequence of the dG-tailed 5' RACE product showing the chbC transcriptional start site (enlarged font). Sequences were determined using the anti-sense primer BBB04 5' RACE R2.Back to article page