Figure 4
From: Chitobiose utilization in Borrelia burgdorferi is dually regulated by RpoD and RpoS
Mutation of rpoS delays chitobiose utilization. Growth of B. burgdorferi strains (A) B31-A (WT), (B) A74 (rpoS mutant) and (C) WC12 (rpoS complemented mutant) in BSK-II lacking GlcNAc (open circle) and supplemented with 1.5 mM GlcNAc (closed circle), or high (closed triangle, 75 μM or 150 μM) or low (open triangle, 5 μM or 15 μM) concentrations of chitobiose. Late-log phase cells were diluted to 1.0 × 105 cells ml-1 in the appropriate medium, incubated at 33°C, and enumerated daily as described in the Methods. This is a representative experiment that was repeated three times.