|
Genes
|
Killed Pg MOI:100 4 h
|
Killed Pg MOI:100 24 h
|
Live Pg MOI:100 4 h
|
Live Pg MOI:100 24 h
|
|---|
|
LTA
|
4.7 ± 3.4**
|
0.4 ± 0.1***
|
1.1 ± 0.8*
|
3.8 ± 1.2**
|
|
TNF
|
0.4 ± 0.01
|
2.0 ± 0.01**
|
2.1 ± 0.2***
|
1.6 ± 0.1***
|
|
NFKB1
|
0.5 ± 0.01
|
1.4 ± 0.03
|
0.9 ± 0.1*
|
1.5 ± 0.05*
|
|
TRADD
|
0.8 ± 0.01
|
1.5 ± 0.3**
|
0.9 ± 0.2
|
3.4 ± 0.1***
|
|
BID
|
0.7 ± 0.02
|
1.6 ± 0.1***
|
0.9 ± 0.1*
|
3.1 ± 0.08***
|
|
CASP9
|
1.9 ± 0.7**
|
0.6 ± 0.2*
|
2.4 ± 1.1**
|
2.2 ± 0.2**
|
|
CASP3
|
1.2 ± 0.02*
|
1.0 ± 0.01
|
1.2 ± 0.1*
|
2.2 ± 0.4***
|
|
BAX
|
1.5 ± 0.5*
|
1.0 ± 0.08
|
1.2 ± 0.01
|
1.7 ± 0.8**
|
|
BCL2
|
0.9 ± 0.02**
|
0.7 ± 0.02**
|
0.9 ± 0.1**
|
1.2 ± 0.7*
|
|
FADD
|
1.2 ± 0.01
|
1.0 ± 0.01
|
1.2 ± 0.1*
|
1.3 ± 0.05**
|
|
RELA
|
0.9 ± 0.03**
|
1.2 ± 0.05**
|
1.1 ± 0.08*
|
1.5 ± 0.1***
|
|
ENDO-G
|
0.9 ± 0.01
|
1.0 ± 0.01
|
1.0 ± 0.1
|
1.3 ± 0.1**
|
|
CHUK
|
0.9 ± 0.06*
|
1.2 ± 0.08**
|
1.1 ± 0.1*
|
1.2 ± 0.3**
|
|
CASP8
|
0.9 ± 0.01**
|
1.0 ± 0.07
|
1.0 ± 0.1
|
1.1 ± 0.1**
|
|
FASLG
|
1.3 ± 0.02
|
1.3 ± 0.02**
|
1.5 ± 0.1**
|
0.9 ± 0.2**
|
|
DFFB
|
1.3 ± 0.03**
|
1.0 ± 0.1
|
1.2 ± 0.2*
|
0.8 ± 0.01
|
- HGECs were challenged with live or heat-killed P. gingivalis 33277 at MOI:100 for 4 and 24 hours. Negative control was unchallenged HGECs in media. The data shown represent log-fold differences in gene expression (means ± SD) between the respective sample and the negative control. A value of 1 indicates no change, less than one indicates down-regulation and greater than one, up-regulation (*P < 0.05 ** P < 0.01, *** P < 0.001)
