Porphyromonas gingivalis induce apoptosis in human gingival epithelial cells through a gingipain-dependent mechanism

Table 1 Apoptotic markers included in the qPCR-Array shown in Fig. 1.

Genes	Killed Pg MOI:100 4 h	Killed Pg MOI:100 24 h	Live Pg MOI:100 4 h	Live Pg MOI:100 24 h
LTA	4.7 ± 3.4**	0.4 ± 0.1***	1.1 ± 0.8*	3.8 ± 1.2**
TNF	0.4 ± 0.01	2.0 ± 0.01**	2.1 ± 0.2***	1.6 ± 0.1***
NFKB1	0.5 ± 0.01	1.4 ± 0.03	0.9 ± 0.1*	1.5 ± 0.05*
TRADD	0.8 ± 0.01	1.5 ± 0.3**	0.9 ± 0.2	3.4 ± 0.1***
BID	0.7 ± 0.02	1.6 ± 0.1***	0.9 ± 0.1*	3.1 ± 0.08***
CASP9	1.9 ± 0.7**	0.6 ± 0.2*	2.4 ± 1.1**	2.2 ± 0.2**
CASP3	1.2 ± 0.02*	1.0 ± 0.01	1.2 ± 0.1*	2.2 ± 0.4***
BAX	1.5 ± 0.5*	1.0 ± 0.08	1.2 ± 0.01	1.7 ± 0.8**
BCL2	0.9 ± 0.02**	0.7 ± 0.02**	0.9 ± 0.1**	1.2 ± 0.7*
FADD	1.2 ± 0.01	1.0 ± 0.01	1.2 ± 0.1*	1.3 ± 0.05**
RELA	0.9 ± 0.03**	1.2 ± 0.05**	1.1 ± 0.08*	1.5 ± 0.1***
ENDO-G	0.9 ± 0.01	1.0 ± 0.01	1.0 ± 0.1	1.3 ± 0.1**
CHUK	0.9 ± 0.06*	1.2 ± 0.08**	1.1 ± 0.1*	1.2 ± 0.3**
CASP8	0.9 ± 0.01**	1.0 ± 0.07	1.0 ± 0.1	1.1 ± 0.1**
FASLG	1.3 ± 0.02	1.3 ± 0.02**	1.5 ± 0.1**	0.9 ± 0.2**
DFFB	1.3 ± 0.03**	1.0 ± 0.1	1.2 ± 0.2*	0.8 ± 0.01

HGECs were challenged with live or heat-killed P. gingivalis 33277 at MOI:100 for 4 and 24 hours. Negative control was unchallenged HGECs in media. The data shown represent log-fold differences in gene expression (means ± SD) between the respective sample and the negative control. A value of 1 indicates no change, less than one indicates down-regulation and greater than one, up-regulation (*P < 0.05 ** P < 0.01, *** P < 0.001)

ISSN: 1471-2180