Figure 9

Focused qPCR-Array consisting of 86 genes relevant to inflammation and apoptosis. HGECs were challenged with live or heat-killed P. gingivalis 33277 at MOI:100 for 4 and 24 hours. Negative control was unchallenged HGECs in media. The mRNA fold change between each sample and the negative control was calculated based on the ΔΔCt method and Log10 fold-increase was used to generate the heatmap using MeV v4.1 release software and hierarchical clustering with Pearson correlation. (A) represents a heatmap of the 86 genes and (B) represents specific apoptotic markers with color coding: Magenta (up-regulated genes) to Green (down-regulated genes). The apoptotic markers in (B) and the fold differences are shown in Table 1.