Heatflow data (column A) and resultant cumulative heat curves (column B) for the IMC determinations of the MICs of ampicillin, piperacillin and aztreonam for E. coli ATCC25922 using IMC. Experiments were performed in cation-adjusted Mueller-Hinton II broth at 37°C. t1, t2, t4: t
for 0 mg l-1 antibiotic; t3: t
for 2 mg l-1 piperacillin; t5: t
for 0.125 mg l-1 aztreonam. Blank is medium alone. Curves are the mean of three replicates.