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Figure 3 | BMC Microbiology

Figure 3

From: Molecular characterization of a fungal gene paralogue of the penicillin penDE gene of Penicillium chrysogenum

Figure 3

Characterization and expression of the ial gene and in vivo activity of the IAL in P. chrysogenum. (A) Southern blotting carried out with genomic DNA extracted from the npe-10-AB·C and Wis54-1255 strains and digested with Hind III. The ial gene was used as probe. (B) HPLC analysis confirming the production of IPN by the npe10-AB·C strain. (C) Chromatogram showing the lack of 6-APA production in the npe10-AB·C strain. (D) Chromatogram showing the lack of benzylpenicillin production in the npe10-AB·C strain. (E) Northern blot analysis of the ial gene expression in npe-10-AB·C and Wis54-1255 strains. Expression of the β-actin gene was used as positive control.

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BMC Microbiology

ISSN: 1471-2180

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