Genotype | Cells | Septa | Polar | % Polar | Phenotype |
---|
DH5α | 867 | 229 | 6 | 3 | WT |
HL1 | 991 | 216 | 119 | 55 | Min- |
HL1(Plac::EcMinDE) | 974 | 232 | 3 | 1 | WT |
HL1(Plac::AtMinD) | 863 | 161 | 11 | 6 | WT |
HL1(Plac::gfp-AtMinD) | 1081 | 219 | 10 | 5 | WT |
HL1(Plac::AtMinD-gfp) | 943 | 137 | 17 | 12 | WT like |
- Shown above is the division phenotype analysis of E. coli cells with different genotypes. EcMinDE was induced with 20 μM IPTG, AtMinD and its GFP fusion proteins were induced with 50 μM IPTG. Cells: the total number of cell examined; Septa: the total number of septa counted; Polar: the number of septa which were misplaced at or near a cell pole; % Polar: the percentage of septa which were misplaced at or near a cell pole. Min-, minicell phenotype. WT, most of the cells have a normal size and no cell or only a small part of the cells are minicells or long filaments.