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Table 2 Analysis of the cell division phenotype

From: A plant MinD homologue rescues Escherichia coli HL1 mutant (ΔMinDE) in the absence of MinE

Genotype

Cells

Septa

Polar

% Polar

Phenotype

DH5α

867

229

6

3

WT

HL1

991

216

119

55

Min-

HL1(Plac::EcMinDE)

974

232

3

1

WT

HL1(Plac::AtMinD)

863

161

11

6

WT

HL1(Plac::gfp-AtMinD)

1081

219

10

5

WT

HL1(Plac::AtMinD-gfp)

943

137

17

12

WT like

  1. Shown above is the division phenotype analysis of E. coli cells with different genotypes. EcMinDE was induced with 20 μM IPTG, AtMinD and its GFP fusion proteins were induced with 50 μM IPTG. Cells: the total number of cell examined; Septa: the total number of septa counted; Polar: the number of septa which were misplaced at or near a cell pole; % Polar: the percentage of septa which were misplaced at or near a cell pole. Min-, minicell phenotype. WT, most of the cells have a normal size and no cell or only a small part of the cells are minicells or long filaments.