Localization of AtMinD in Arabidopsis and E. coli with a GFP tag. (A to C) AtMinD-GFP transiently expressed in an Arabidopsis protoplast. Arrows denote the localization of GFP in chloroplasts. (D and G) AtMinD-GFP expressed in E. coli HL1 mutant. (E and H), GFP-AtMinD expressed in E. coli HL1 mutant. (F and I) GFP-EcMinD expressed in E. coli HL1 mutant, (J and K) GFP-EcMinC and AtMinD expressed in E. coli RC1 mutant, (L and M) GFP-EcMinC expressed in E. coli RC1 mutant, (N) Immuno blot analysis. AtMinD-GFP, GFP-AtMinD and GFP-EcMinD were expressed in the HL1 mutant; GFP-EcMinC was expressed in the RC1 mutant with AtMinD. All the cells were grown with 20 or 50 μM IPTG. (A, D, E, F, J and L), GFP; (B), Chlorophyll; (C) Overlay; (G, H, I, K and M), DIC. Bars are 5 μm.