Figure 7
Effects of SSPLA 2 effectors on the yeast budding cycle. Yeast cells grown, harvested, synchronized and selected by filtration as described in Methods were induced to re-enter the budding cycle in a basal medium with glucose at pH 7.2 and incubated at 25°C in the presence and absence of arachidonic acid (40 μM), AACOCF3 (100 μM; Nonadeca-4,7,10,13-tetraenyl-trifluoro-methyl ketone) and isotetrandrine (50 μM; 6,6',7,12-tetra methoxy-2,2'-dimethyl-berbaman). All values are given as the average percentage ± one SD of at least three independent experiments. The Student's t test was used to determine the statistical significance of the data at a 95% confidence level. Values that differ significantly from those of the control at 95% confidence level are marked with an asterisk.