Figure 5

Amino acid sequence alignments of SSPLA ₂ with other PLA ₂ homologues. The S. schenckii SSPLA₂ was aligned to other PLA₂ fungal homologues as described in Methods. The fungal PLA₂ used for the alignment were: E. nidulans (PLA₂), M. grisea (hypothetical protein), N. crassa (PLA₂), C. globosum (hypothetical protein), P. anserina (hypothetical protein) and G. zeae (PLA₂). The alignment was done using MCOFFEE and visualized using the program GeneDoc. Only the catalytic core of these proteins is shown in this alignment, from amino acids 192 to 611 (in reference to the multiple alignment position). The black shading with white letters indicates 100% identity, gray shading with white letters indicates 75–99% identity, gray shading with black letters indicates 50–74% identity.