| PLASMID |  |
---|---|---|
Name | Description | Reference |
pTV1-OK | Temperature sensitive plasmid for the delivery of Tn917. 11 kb. EryR KanR. | [33] |
pNZ272 | Lactococcal beta-glucuronidase (gusA) transcriptional fusion vector. 4.6 kb. CmR. | [34] |
pIMC | Site-specific Listerial integrative vector. High-level CmR from Phelp driven expression. 4.6 kb. CmR. | This study |
pIMK3 | Site-specific Listerial integrative vector. High level IPTG controlled gene expression. 7.5 kb. KanR. | [27] |
pIMC3kan | Site-specific Listerial integrative vector. IPTG controlled expression of aphA-III ex pTV1-OK. CmR | [33] This study |
pIMC3gus | Site-specific Listerial integrative vector. IPTG controlled expression of gusA ex pNZ272. CmR | [34] This study |
pIMC3ery | Site-specific Listerial integrative vector. IPTG controlled expression of ermAM ex pTV1-OK. CmR | [33] This study |
pIMC3tet | Site-specific Listerial integrative vector. IPTG controlled expression of tetM ex CH919. CmR | [35] This study |
 | BACTERIA |  |
Listeria monocytogenes | Â | Â |
EGDe | Wild type 1/2a strain. Genome sequenced. | [9] |
10403S | Wild type 1/2a strain. Genome sequenced. Broad Institute (MIT). | [36] |
F2365 | Wild type 4b strain. Genome sequenced. | [10] |
EGDe::pIMC3kan | EGDe transformed with pIMC3kan integrated at the tRNAARG. | This study |
EGDe::pIMC3gus | EGDe transformed with pIMC3gus integrated at the tRNAARG. | This study |
EGDe::pIMC3ery | EGDe transformed with pIMC3ery integrated at the tRNAARG. | This study |
EGDe::pIMC3tet | EGDe transformed with pIMC3tet integrated at the tRNAARG. | This study |
10403S::pIMC3ery | 10403S transformed with pIMC3ery integrated at the tRNAARG. | This study |
F2365::pIMC3tet | F2365 transformed with pIMCtet integrated at the tRNAARG. | This study |
Other Bacteria | Â | Â |
DH10B | Wild type Escherichia coli. Used for routine cloning. | Invitrogen |
CH919 | MG1363 Lactococcus lactis subsp. lactis containing Tn919. | [36] |
 | OLIGONUCLEOTIDE |  |
Name | 5'-3' a | RE site |
108 Phelp B REV | CATGGGTTTCACTCTCCTTCTAC | Â |
181 CAT C FWD | AAATGTAGAAGGAGAGTGAAACCCATGAACTTTAATAAAATTGATTTAGAC | Â |
203 MCS FWD | AATTAACCCTCACTAAAGGGAAC | Â |
204 MCS REV | GACGTCGTAATACGACTCACTATAGGGC | Â |
205 Back FWD | AGTGAGTCGTATTACGACGTCCCAGGGCTTCCCGGTATCAAC | Â |
206 Phelp A FWD | ATCCCATTATGCTTTGGCAGTTTATTC | Â |
209 CAT D REV | ATAATGAGACAGAATTATGATGATCATATGTCAACTAACGGGGCAGG | Â |
211 Back REV | CATATGATCATCATAATTCTGTCTCATTATATAAC | Â |
373 gusA FWD | TATACCATGG TACGTCCTGTAGAAACCCCAACC | Nco I |
374 gusA REV | ATATCTGCAG TTATTGTTTGCCTCCCTGCTGCGG | Pst I |
385 aphA3 FWD | TAATCCATGG CTAAAATGAGAATATCACCGGAATTG | Nco I |
386 aphA3 REV | AAGCCTGCAG TTAAAGCTTTTTAGACATCTAAATCTAGG | Pst I |
387 ermAM FWD | ATATCCATGG ACAAAAATATAAAATATTCTCAAAAC | Nco I |
388 ermAM REV | ATATCTGCAG TTATTTCCTCCCGTTAAATAATAGATAAC | Pst I |
408 tetM FWD | ATATCCATGG AAATTATTAATATTGGAGTTTTAGC | Nco I |
409 tetM REV | ATATCTGCAG TTAAGTTATTTTATTGAACATATATCG | Pst I |